Complement Factor H antibody

Catalog No. ABIN181114

This Mouse Monoclonal antibody specifically detects Complement Factor H in WB, FACS, EIA and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Complement Factor H antibody (ABIN181114)

Target: Complement Factor H (CFH)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Complement Factor H antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 10-10-14

Product Details anti-Complement Factor H Antibody

Specificity: This antibody recognises Complement Factor H which exists in 2 forms.

Characteristics: Synonyms: CFH, HF, HF1, HF2, H factor 1

Purification: Affinity Chromatography on Protein A.

Immunogen: Purified human factor H from serum.

Isotype: IgG1

Application Details

Application Notes: ELISA. Western Blot. Flow Cytometry. Immunohistochemistry on Frozen Sections. Recommended Positive Control: Kidney from post streptoccal glomerulonephritis patients.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Borate buffered saline pH 8.4 containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Complement Factor H

Target: Complement Factor H (CFH)

Background: Complement factor H exists in 2 forms, the most common form, of 150 kDa, and the less common form of 43 kDa. Factor H is secreted by the liver into the blood serum. It is important in regulating the complement pathway, preventing unnecessary inflammation which can damage the host tissue. Factor H functions as a cofactor in the inactivation of C3b by factor I. It makes C3b susceptible to cleavage by factor I, resulting in iC3b. Factor H also inhibits the formation of the C3bBb complex (C3 convertase) and increases the rate of dissociation of both C3 convertase and the (C3b)NBB complex (C5 convertase). This prevents these components of the classical and of alternative complement pathways from forming a positive feedback loop. Mutations in factor H are associated with hemolytic-uremic syndrome, age-related macular degeneration, membranoproliferative glomerulonephritis (MPGN) type II and chronic hypocomplementemic nephropathy.Synonyms: CFH, H factor 1, HF, HF1, HF2

Gene ID: 3075

UniProt: P08603

Pathways: Complement System, Cellular Response to Molecule of Bacterial Origin